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Effect of LY294002 on Akt phosphorylation in LMP2A-expressing Ramos and HSC-39 cells. LMP2A-expressing Ramos (A) and HSC-39 (B) cells were cultured for 1 h without serum. Cells were incubated for 1 h with LY294002 (50 μM). Levels of P-Akt (60 kDa) were determined by immunoblotting. The lower panels show equal loading of proteins and the expression of total Akt (T-Akt) (60 kDa).
