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. 2013 Jun 27;9(6):e1003450. doi: 10.1371/journal.ppat.1003450

Figure 3. Japanin binds specifically to DC.

Figure 3

(A) Monocyte-derived dendritic cells, or (B and C) human blood PBMC were incubated with 100 ng/ml Japanin-DyLight 649 (filled histograms) or 340 ng/ml OmCI-DyLight 649 (open histograms), incubated on ice for 1 hour, and washed. Binding was assessed by flow cytometry. In B, major PBMC subsets are defined by surface molecule expression. In C, blood DC are defined as CD14−HLA-DR+lineage−CD16− then further subdivided according to CD1c, CD11c, CD123 and CD141 expression. For the complete gating strategy, see figure S4. Results shown are representative of those from 6 (A) and 4 (B and C) experiments with cells from different donors.