Figure 9. Therapeutic Cyld siRNA treatment protects WT mice from lethal listeriosis.

(A) WT and Cyld^−/− mice were i.v. infected with 5×10⁵ Lm. Infected mice were treated as indicated 24 h p.i. At day 5 p.i., proteins were isolated from the liver (n = 3 per experimental group) and CYLD, pSTAT3 fibrin, and GAPDH production was analysed by WB. (B, C, D) Quantification of CYLD (B), pSTAT3 (C) and fibrin (D) (± SD) was performed from WB data of the indicated groups. The results present 3 mice per experimental group. (E) The survival rates of Lm-infected untreated Cyld^−/− and Cyld siRNA-treated WT mice were significantly were increased as compared to untreated WT mice (p<0.01 for Cyld^−/− vs. WT mice, p<0.05 for Cyld siRNA treated WT vs. WT mice). Ten mice per group were analysed until day 10 p.i. (F) CFUs were determined in the liver at day 5 p.i. Five mice were analysed per group and data show the mean ± SD (* p<0.05, ** p<0.01). In (A–G) data from one of two representative experiments are shown. (G) Macroscopic analysis showed haemorrhage of untreated and control siRNA-treated infected WT mice. Haemorrhage was reduced in WT mice treated with Cyld siRNA and was absent from untreated Cyld^−/− mice.