FIG. 8.

Mutation of the NF-κB-like binding site at −52/−43 reduces bfl-1 promoter transcriptional activation upon engagement of CD40 receptor. DG75 cells were transfected with 2 μg of reporter construct (indicated underneath the graph) and then cultured for 24 h on either CD40lig-L cells or CD32-L cells, which express human CD40 ligand and human CD32/Fc-gRII, respectively. DG75 cells were then harvested and analyzed for luciferase activities which were normalized for transfection efficiency (based on β-galactosidase activity measured from cotransfected pCMVlacZ reporter, which was included in all transfections). The absolute luciferase values obtained for 3Enh-Luc, pGa(−129/−34), and −129/+81-Luc after transfection and cocultivation with CD40lig-L cells were set at 100%. Percent values for pGa(−129/−34mκB) and −129/+81mκB are given relative to pGa(−129/−34) and −129/+81, respectively.