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Plaque formation of ts2 at 39.7°C is restored by the expression of hVRK1, mVRK1, and wt B1, but not hCK1α. Confluent 35-mm-diameter dishes of BSC40 cells were infected with 200 PFU of the viruses indicated/plate and incubated at either 31.5 or 39.7°C for 2 days. Cells were then fixed and stained with 3.7% formaldehyde-0.1% crystal violet to visualize the viral plaques.
