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. 2013 Feb 28;7(7):1286–1298. doi: 10.1038/ismej.2013.20

Figure 1.

Figure 1

Microbial community analyses of groundwater samples collected during the Fe(III)-reducing phase of the 2010 field experiment. (a) Estimates of bacterial and protozoan cell numbers calculated from 16S rRNA and protozoan β-tubulin (b2t) gene copy numbers estimated by qPCR (Pearson's correlation, r=0.90, P=0.015). (b) The number of Breviata β-tubulin and Geobacter citrate synthase (gltA) gene copies per μg total DNA (r=0.51, P=0.02). (c) Percentage breakdown of bacterial 16S rRNA gene sequences detected in clone libraries constructed from genomic DNA extracted from groundwater. (d) Percentage breakdown of protozoan 18S rRNA gene sequences detected in clone libraries constructed from genomic DNA extracted from groundwater.