Table 2. Dynamics of fixation of the vsrA, prhI and p¹¹² hrpG mutations in evolved populations.

Mutations	Populations
vsrA	M2	M3	M4	M5	M6
Number of reads	3191	2808	4509	6210	6122
% of mutated sequences	0.9	11.8	21.2	89.1	99.6
prhI	N10	N11	N12	N13
Number of reads	4004	2200	1480	2626
% of mutated sequences	0	0.04	85.2	99.2
p112hrpG	S7	S8	S9	S10
Number of reads	612	1004	943	1165
% of mutated sequences	0.4	0.1	89.9	97.2

Mutation frequencies were calculated by resequencing regions surrounding the mutations using GS FLX 454.

Mutation frequencies in populations N10, N11, S7 and S8 were below the level of detection of the method.