Table 2. Sequencing results.
| Subject | Groups | 16S DNAa | HC (LC) OTUsa | 16S RNAb | HC (LC) OTUsb | ITS DNAc | OTUsc |
|---|---|---|---|---|---|---|---|
| 2 | HC+LC | 12 755 | 358 (53) | 424 | − (48) | — | — |
| 15 | HC+LC | 11 714 | 92 (15) | — | 18 264 | 71 | |
| 16 | LC | 458 | − (46) | 336 | − (63) | — | — |
| 26 | LC | 189 | − (51) | 184 | − (39) | — | — |
| 31 | LC | 254 | − (56) | — | — | — | — |
| 33 | HC+LC | 9649 | 214 (44) | 12 847 | 241 (30) | 15 856 | 72 |
| 34 | HC+LC | 10 676 | 105 (17) | 14 071 | 86 (13) | — | — |
| 38 | HC+LC | 11 570 | 284 (42) | 513 | − (42) | 19 589 | 81 |
| 41 | LC | — | — | 343 | − (41) | — | — |
| 42 | LC | 321 | − (49) | — | — | — | — |
| 44 | HC+LC | 10 786 | 282 (39) | 14 445 | 276 (37) | 19 397 | 57 |
| 48 | LC | 548 | − (37) | 283 | − (61) | — | — |
| 49 | LC | — | — | 264 | − (42) | — | — |
| 50 | HC+LC | 11 096 | 215 (32) | 11 660 | 208 (33) | 5766 | 32 |
| 52 | HC+LC | 9973 | 172 (38) | 12 419 | 213 (35) | — | — |
| 56 | HC+LC | 8599 | 183 (42) | 301 | − (34) | — | — |
| 64 | HC+LC | 9547 | 26 (8) | 283 | − (13) | 10 737 | 42 |
| 67 | HC+LC | 4809 | 95 (32) | 239 | − (31) | — | — |
| 68 | LC | 138 | − (26) | 316 | − (40) | — | — |
| 76 | HC+LC | 5143 | 165 (48) | 298 | − (48) | — | — |
| 77 | HC+LC | 10 392 | 239 (43) | 430 | − (47) | 16 407 | 61 |
| 84 | LC | 286 | − (18) | 124 | − (17) | — | — |
| 94 | LC | 197 | − (27) | — | — | — | — |
| 96 | HC+LC | 8118 | 114 (25) | 110 | − (14) | 11 422 | 19 |
| 97 | HC+LC | 10 577 | 204 (30) | 271 | − (39) | 16 582 | 51 |
Abbreviations: HC, high-coverage; ITS, internal transcribed spacers; LC, low-coverage; OTU, operational taxonomic unit.
a
16S rRNA gene PCR amplicon sequence reads.
b
16S rRNA transcript RT-PCR amplicon sequence reads.
c
Internal Transcribed Spacer PCR amplicon sequence reads. Numbers refer to reads that passed binning by sample barcode, filtering for length, quality, ambiguous base pairs, homopolymers and chimeras as described in the Materials and methods section.