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SRM chromatogram of a blank urine sample spiked at a level of 12.5 μg/L ZEN-14-GlcA. It is obvious that the transition m/z 493 [M-H]-→131 results in a far better signal-to-noise ratio than the other product ions despite its lower absolute abundance. Hence, this fragment should be chosen as a quantifier ion. In addition, the more intense transitions comprise many interferences which mimic ZEN-14-GlcA and thus potentially may lead to false-positive results
