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FACS analysis of lymphoma vascular cell populations. (A) Quad-gate analysis of the EL4 tumor-associated cells. Murine EPCs (mCD45−VEGFR2+CD117+), mature ECs (mCD45−VEGFR2+CD31+), murine PPCs (mCD45−CD140b+CD117+), and mature PCs (mCD45−CD140b+NG2+) are shown. Blue-dotted squares outline individual quad-gates for EPCs, PPCs, ECs, and PCs. (B) Quantification of stromal cell populations in EL4 tumors treated with imatinib vs PBS. *P < .05 compared with control.
