Figure 2.

Mitochondrial swelling induced by CaCl₂. (A) Typical experiment showing the scattered light absorbance traces of Ca²⁺-induced swelling in response to addition of CaCl₂ for heart mitochondria isolated from rats transduced with shRNA-NHE1 in the presence and absence of cariporide. The shRNA-NHE1 transduction significantly inhibited Ca²⁺-induced mitochondrial swelling compared with SCR-transduced animals, (as previously shown in Figure 1). Cariporide and Cyclosporine attenuated the decrease in light scattering in SCR-transduced rats and did not affect the decrease in light scattering in shRNA-NHE1-transduced rats. These results indicate that the NHE1 participates in the mechanism by which the Ca²⁺ induces MPTP opening and swelling. (B) Scheme showing the absence of mitochondrial swelling and MPTP opening induced by Ca²⁺ when NHE1 is silencing. Modified from Villa-Abrille et al. (2011).