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. 2010 Mar 25;298(6):G908–G915. doi: 10.1152/ajpgi.00510.2009

Fig. 2.

Fig. 2.

EphB4 activates VEGF promoter. A: LX2 cells cotransfected with Renilla control and full-length human VEGF reporter luciferase construct (pVEGF) showed an increased luciferase activity upon stimulation with both EphrinB2 (2 μg/ml) and EphB4 (2 μg/ml) chimeras, with EphB4 stimulation achieving a statistically significant effect (n = 3 separate experiments, each in triplicate, *P < 0.05). B: knockdown of EphrinB2 or EphB4 with their respective siRNA resulted in a 40–50% reduction in luciferase activity compared with control siRNA, whereas stimulation of siRNA-knockdown cells with activating chimera reconstituted basal promoter activity (n = 3 separate experiments, each in triplicate, *P < 0.05). C: stimulation of LX2 cells by EphB4 or EphrinB2 chimeras significantly increased KLF2 mRNA levels as assessed by quantitative real-time PCR (n = 3 separate experiments, each in triplicate, *P < 0.05).