Figure 5.

Cx29 immunofluorescence analyses and microinjections of differentiated Oli-neu cells. (A) Distribution of the Cx29 protein mostly in plasma membranes (arrows) applying Cx29-specific and Cy3 coupled secondary antibodies in relation to the cellular nuclei after DAPI staining. (B) Omitting primary Cx29 antibodies excluded cross reactivity of Cy3 antibodies with Oli-neu cells. (C and D) Two examples of Oli-neu cells microinjected with neurobiotin and stained thereafter (arrow) (C) No neurobiotin transfer into both cells directly attached below was detectable. (D) After filling one cell (large arrow), neurobiotin readily spreads into the three protrusions but did not migrate further into adjacent cells at their ends (small arrows). (E) Combined UV light- and trans-illumination identified the Etbr-microinjected Oli-neu cell (arrow). (F) Only the Etbr filled soma and the protrusions of the injected cell are faintly visible after 7 min (arrows).