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. Author manuscript; available in PMC: 2013 Jun 28.
Published in final edited form as: J Immunother. 2010 Jan;33(1):83–91. doi: 10.1097/CJI.0b013e3181b8f421

FIGURE 1.

FIGURE 1

HLA-A*0201 stabilization assay comparing peptides derived from epidermal growth factor receptor (EGFR). Stabilization of cell surface HLA-A*0201 molecules was determined after incubation of T2 cells with EGFR or flu peptides at various concentrations (ranging from 10 to 0.01 μg/mL) as described in Materials and Methods. Flow cytometry staining was performed by incubating 5 × 105 loaded cells with anti-HLA class 1 mAb W6/32, then fluorescein isothiocyanate-labeled secondary mAb for 30 minutes each at 4°C. Mean fluorescence intensity detected by flow cytometry is shown, reflecting results representative of 3 similar experiments.