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. Author manuscript; available in PMC: 2013 Jun 28.
Published in final edited form as: J Immunother. 2010 Jan;33(1):83–91. doi: 10.1097/CJI.0b013e3181b8f421

FIGURE 4.

FIGURE 4

CTL recognition of naturally processed EGFR853 – 861 on SCCHN cells. EGFR853 – 861 CTL recognition of naturally processed peptide expressed by SCCHN cell lines (A) SCC-4 and (B) PCI-30 using IFN-γ ELISPOT assays was tested using IFN-γ ELISPOT assays. IFN-γ release was blocked by an anti-HLA class 1 mAb. No reactivity was observed using HLA-A*0201− SCCHN cells (not shown). Incubation of SCCHN cells with IFN-γ (100 IU/mL for 72 h at 37°C) increased CTL recognition, but not EGFR expression (Fig. 4C). CTL indicates cytotoxic T lymphocyte; EGFR, epidermal growth factor receptor; ELISPOT, enzyme-linked immunosorbent spot; SCCHN, head and neck squamous cell carcinoma.