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Comparing the effect of pulse train with pulse pair: Change in the relative fluorescence intensity of DAPI versus Mito-tracker Red under one-color (a) pulse-pair excitation and (b) pulse train excitation. (c) Corresponding fluorescence suppression image of Mito-tracker Red relative to DAPI as a function of time delay between the trains of pulse under one-color excitation (no suppression for pulse pair excitation for this dye pair).
