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. Author manuscript; available in PMC: 2013 Aug 3.
Published in final edited form as: Circ Res. 2012 Jun 22;111(4):469–481. doi: 10.1161/CIRCRESAHA.112.266361

Figure 3. Effects of CaSR modulators on [Ca2+]cyt in IPAH-PASMC.

Figure 3

A. Representative traces (left panels) and summarized data (means±SE, right panel) showing the effect of spermine (3 mM), a CaSR agonist, on [Ca2+]cyt in the absence (0Ca) and presence (2.2Ca) of extracellular Ca2+ (2.2 mM) in normal (n=30) and IPAH PASMC (n=52). **P<0.01 vs. normal PASMC (spermine/0Ca); ##P<0.01 vs. normal PASMC (spermine/2.2Ca). B and C. Representative records of [Ca2+]cyt changes (left panels) and summarized data (means means±SE, right panels) of the stimulatory effect of R568 (1 μM, n= 63, B), a positive allosteric modulator of CaSR, and the inhibitory effect of NPS-2143 (10 μM, n=120, C), a negative allosteric modulator of CaSR, on the 0.5-mM extracellular Ca2+-induced [Ca2+]cyt increases in IPAH-PASMC. **P<0.01 vs. Control IPAH-PASMC (without treatment with vehicle, R568 or NPS 2143).