Table 1.

Stoichiometry and EC₅₀ of reaction between DPPH free radicals and chitosan–GA under experimental conditions

Conditions	Stoichiometrya	EC50 (mg mL−1)
Room temperature	0.11 ± 0.01	0.137 ± 0.004
Reduced chitosan–GAb	0.11 ± 0.03	0.138 ± 0.007
Incubated for 30 minc	0.08 ± 0.03	0.144 ± 0.009
Incubated for 60 minc	0.10 ± 0.01	0.141 ± 0.007

All values reported are mean ± SD, n = 5.

^aThe number of DPPH free radicals scavenged by a molecule of antioxidant.

^bAntioxidant samples were reacted with Zn⁰ metal 6 mol equiv to antioxidant.

^cAntioxidant samples were incubated at 100 °C.