Table 1.
Region | Positions | Sequence | MFEa | Controla,b | MFEDc | Z-score |
---|---|---|---|---|---|---|
1 | 1147–2467 | WT | −97.0 | −90.5 | 9.10% | −4.2 |
F1 | −88.5 | −91.6 | −0.9% | −0.15 | ||
2 | 3124–4066 | WT | −104.0 | −100.7 | 3.90% | −1.7 |
F2 | −96.2 | −97.7 | −2.6% | −0.01 |
aMinimum free energy on folding (kcal/mol).
bFolding energy of sequence permuted by the NDR scrambling algorithm.
cMFE difference between non-permuted and permuted sequences.