Table 1.

Folding energies of WT and mutated region 1 and region 2 sequences

Region	Positions	Sequence	MFEa	Controla,b	MFEDc	Z-score
1	1147–2467	WT	−97.0	−90.5	9.10%	−4.2
		F1	−88.5	−91.6	−0.9%	−0.15
2	3124–4066	WT	−104.0	−100.7	3.90%	−1.7
		F2	−96.2	−97.7	−2.6%	−0.01

^aMinimum free energy on folding (kcal/mol).

^bFolding energy of sequence permuted by the NDR scrambling algorithm.

^cMFE difference between non-permuted and permuted sequences.