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Increase in DHS signal at the intragenic PRbs after hormone induction. T47D cells were treated with R5020 for 60 min and subjected to DNAse-seq analysis (35). Top and middle: the profiles of DHS signals obtained in the presence and in the absence of R5020, as well as the PRbs obtained by ChIP-seq (22) in the bcl-x gene are shown. Bottom: Quantification of the DHS reads associated with the +2.5, +3.5, +42 and +58 PRbs. The window used in each PRbs was ±50 bp.
