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The general scheme of the organic synthesis procedure. Reagents and conditions: (i) N-hydroxysuccinimide ester of TFA-NH-(CH2)5-COOH, Et3N, DMF; (ii) DMTCl, pyridine; (iii) aqueous NH3 (25%), pyridine; (iv) (a) 9-anthracenecarboxylic acid, N-hydroxybenzotriazole, DCC, CH2Cl2, Et3N, (b) 5(6)-carboxyfluorescein-NHS ester, Et3N, CH2Cl2; and (v) 2-cyanoethyl-N,N,N′,N′-tetraisopropylphosphodiamidite, diisopropylammonium tetrazolide, CH2Cl2.
