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Optimization of recombineering efficiency. (A) Recombination efficiency of recT induced for 0, 1 and 4 h using either pCLTON2-recT and 0.25 mg/l of anhydrotetracyclin or pEKEx3-rect and 0.24 mg/l of IPTG. (B) Recombination efficiency depended on the amount of oligonucleotide added. (C) Recombination efficiency depended on length and strand homology. Data are the means of three experiments, with the standard deviation as indicated by the error bars.
