Figure 5.

MKL1 recruits Brg1 and Brm to activate ET-1 transcription. (A) HVECs were exposed to 1% O₂ and harvested at indicated time points. ChIP assays were performed with indicated antibodies. (B) HVECs were exposed to 1% O₂ for 24 h. Re-ChIP assay was performed with indicated antibodies. (C) HVECs were transfected with siMKL1 or SCR followed by exposure to 1% O₂. ChIP assays were performed with anti-Brg1 or Brm. (D) A rat ET-1 promoter-luciferase construct was transfected into HVECs with expression constructs for MKL1, Brg1 or Brm as indicated. Data are expressed as RLU. (E) A rat ET-1 promoter-luciferase construct was transfected into HVECs with expression constructs for Brg1 or Brm followed by exposure to 1% O₂. Data are expressed as RLU. (F) HVECs were transfected indicated siRNA followed by exposure to 1% O₂. ET-1 mRNA and protein levels were measured by qPCR and ELISA.