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In vitro responses of probes 2, 4 and control 6 to human UNG2 enzyme. (A) Time courses of response of probes at 480 nm. (B) Spectral changes in response of probe 2 over the same time course. Excitation = 340 nm. Condition: [hUNG2] = 9.7 mg/ml (90 nM); [probe] = 4 μM; 37°C, excitation 340 nm.
