Table 2.

In vitro enzyme substrate parameters of selected probes with bacterial and human uracil–DNA glycosylases

Enzyme	Substrate	Km(app) (μM)	kcat(app) (min−1)	kcat/Km (min−1 μM−1)
E. coli UDG	5′-AAUYUAA	0.21	2040	9710
E. coli UDG	5′-AAUYYUAA	0.30	882	2940
hSMUG1	5′-AAUYYUAA	0.82	35	43
hUNG2	5′-AAUYYUAA	4.67	4.6	0.99