Fig. 2.

Effects of Iso stimulation on the internalization of β₁-AR into RE in COS-1 cells. β₁-AR cells were plated onto glass coverslips. Cells were incubated with or without 10⁻⁶ M Iso for 30 min in the presence of anti-HA Ab. Cells were then fixed with 4 % paraformaldehyde followed by 0.1 % Triton X-100 (for 10 min in each step). Anti-TfnR Ab was added and incubated for 1 h at room temperature. Abs against HA or TfnR were probed with Alexa546-labeled anti-rabbit or Alexa488-labeled anti-mouse Abs, respectively. A laser scanning microscope (LSM500) was used to capture the images (upper panel images without Iso; lower panel images with Iso for 30 min)