Fig. 4.

Role of SNX27 in β₁-AR recycling after Iso stimulation in COS-1 cells. Coimmunoprecipitation of β₁-AR or ∆C4β₁-AR with SNX27. COS-1 cells were transfected with β₁-AR or ∆C4β₁-AR and SNX27 (myc-tagged). Cells were stimulated with or without 10⁻⁶ M Iso for 10 min, lysed and coimmunoprecipitated with anti-Myc Ab. Precipitated proteins were separated by SDS-PAGE and immunoblotted with anti-β₁-AR or Myc Abs. Lysates (Input) were also probed with the same Abs as well as anti-β-actin Ab. Localization of β₁-AR and SNX27 (b) or EE (c). β₁-AR was transfected into COS-1 cells with or without SNX27 (b, c, respectively). Transfected cells were plated onto glass coverslips, treated with 10⁻⁶ M Iso for 10 and 30 min in the presence of anti-HA Ab and coimmunostained with anti-Myc Ab (b) or anti-EEA1 Ab (c) as described in “Materials and Methods” images were taken with the LSM500 microscope. Bar 20 μm