Fig. 6.

Effects of SNX27 silencing on the subcellular localization of β₁-AR after Iso stimulation in COS-1 cells. Either empty vector (a) or shSNX27 (b) was transfected into β₁-AR cells, and 24 h later, cells were plated onto glass coverslips. After 48 h, cells were treated without or with 10⁻⁶ M Iso for 30 min (−Iso and Iso 30 min, respectively). After incubation, cells were washed to remove Iso and further incubated for 60 min (chase) (Iso 30 and Chase 60 min) to follow the cell surface recovery of β₁-AR. During Iso incubation, anti-HA Abs were added to label cell surface proteins. After incubation, cells were fixed and the internalized β₁-AR (labeled with anti-HA Ab) was costained with anti-TfnR Ab. Bar 20 μm