Fig. 7.

Effects of SNX27 silencing on the degradation of β₁-AR after Iso stimulation in COS-1 cells. Empty vector (a) or shSNX27 (b) was transfected into β₁-AR cells, and 24 h later, cells were plated onto glass coverslips. After 48 h, cells were treated without or with 10⁻⁶ M Iso for 30 min (−Iso and Iso 30 min, respectively). During incubation with Iso, anti-HA Ab was added to label cell surface proteins. After incubation, cells were fixed and the internalized β₁-AR (labeled with anti-HA Ab) was costained with anti-LAMP1 Ab. Bar 20 μm