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. 2013 Jun 12;91(7):825–837. doi: 10.1007/s00109-013-1006-4

Fig. 2.

Fig. 2

a Immunoblot analysis of choroideremia patient fibroblasts (CHM) transduced with AAV2/2-EFS-GFP, AAV2/5-EFS-GFP and AAV2/2-CBA-GFP using GFP antibody and α-tubulin antibody as a loading control. Expression of GFP was analysed 7 days post-transduction. b Immunoblot analysis of CHM transduced with AAV2/2-EFS-REP1, AAV2/5-EFS-REP1 and AAV2/2-CBA-REP1 using 2F1 antibody and α-tubulin antibody as a loading control. Expression of REP1 was analysed 7 days post-transduction. c Immunoblot analysis of choroideremia patient fibroblasts that were untransduced (CHM) and transduced with AAV2/2-CBA-REP1 and control (WT) fibroblasts using 2F1 antibody and α-tubulin antibody as a loading control. Expression of REP1 was analysed 10 days post-transduction. Amount of loaded cell lysate (micrograms) is indicated above each lane. Recombinant human protein (hREP1) was used as a positive control. d Quantification of the GFP signal intensity from the western blot shown in c using ImageJ software. e In vitro prenylation analysis was performed using 5 and 20 μg of cytosolic fractions of the cell lysates isolated from untransduced (white diamond) and transduced with AAV2/2-CBA-GFP (black square) and AAV2/2-CBA-REP1 (white triangle) CHM fibroblasts. f In vitro prenylation analysis was performed using 2, 4, 8 and 16 μg of cytosolic fractions of the cell lysates isolated from untransduced D17 cell (white diamond) and D17 transduced with AAV2/2-CBA-GFP (black square) and AAV2/2-CBA-REP1 (white triangle)