Fig. 3.

a, b Illustration of delivery route for AAV viral vectors by subretinal injection. The subretinal injection was performed through posterior retina using a 10-μl Hamilton syringe and a 30° bevelled 34G needle. c Immunoblot analysis of RPE collected from the eyes of choroideremia mice (Chm ^null/WT) injected with AAV2/2-EFS-REP1, AAV2/5-EFS-REP1 and AAV2/2-CBA-REP1 using 2F1 antibody and α-tubulin antibody as a loading control. Expression of REP1 was analysed 5 weeks post-injection. d–f Histological analysis of the AAV2/2-CBA-GFP-injected mouse eyes 5 weeks post-injection. e Phase contrast image corresponding to d. f Enlargement image of the area boxed in d. Arrows indicate nuclei of rod photoreceptors that express GFP confirming successful transduction. RPE retinal pigment epithelium, ONL outer nuclear layer, IS inner segments, OS outer segments. Bar is 50 μm