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. 2013 Jun 15;2:263. doi: 10.1186/2193-1801-2-263

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© Kragstrup et al.; licensee Springer. 2013

This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Testing for false negative results and other matrix effects. (A) False negative measurements. OD signal from unspiked and spiked plasma samples from two arthritis patients in wells coated with anti-IL-24 specific antibody. (B) Identification and removal of false negative measurements. OD signal from unspiked and spiked plasma samples from the same two arthritis patients in wells coated with anti-IL-24 specific antibody after preincubating samples with a combination of mouse, goat, bovine and human immunoglobulins. (C) Spike recovery assessment in three other arthritis patients. Calculated IL-24 concentrations from unspiked patient samples and patient samples spiked with 800 pg/ml rh IL-24 in wells coated with anti-IL-24 specific antibody after preincubating samples with immunoglobulins. (D) Linearity-of-dilution assessment in three other arthritis patients. Calculated IL-24 concentrations in samples preincubated with immunoglobulin and diluted 4/6, 2/6 and 1/6 in wells coated with anti-IL-24 specific antibody.