Fig. 2.

Impact of gain of IRP1 function on the expression of IRP target genes. Representative western blots of ferroportin (FPN), transferrin receptor 1 (TfR1), and ferritin L (FTL) using protein extracts from the liver, spleen, and duodenum. β-actin was used as a standard. Homozygous males were analyzed; genotypes are indicated above each lane. The histograms represent relative quantification of protein and RNA levels of each IRP target in the three organs. Protein levels were normalized to β-actin, RNA levels to α-tubulin mRNA. Wild type and IRP1* homozygous animals were analyzed; sample size is indicated (n). **p<0.01; ***p<0.001