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. Author manuscript; available in PMC: 2013 Jul 3.
Published in final edited form as: Cell Metab. 2012 Jul 3;16(1):33–43. doi: 10.1016/j.cmet.2012.05.011

Figure 1. Overexpression of Nkx6.1, but not Pdx-1 enhances GSIS and increases VGF expression in primary rat islets.

Figure 1

(A-D) Rat islets were treated with recombinant adenoviruses (AdCMV) expressing Nkx6.1, Pdx-1, VGF or GFP as indicated and analyzed 72 h post-viral treatment. (A, C) Glucose-stimulated insulin secretion was measured by static incubation in media containing 2.5 mM Glc and 16.7 mM Glc for 1 h each. (B) Immunoblot analysis of whole cell lysates. (D) Quantitative RT-PCR was used to measure VGF mRNA levels. (E, F) Rat islets were treated with recombinant adenoviruses expressing Ad-siVGF or Ad-siSCR for 24 h followed by treatment with AdCMV-Nkx6.1 or AdCMV-GFP for an additional 24 h as indicated. Glucose-stimulated insulin secretion was measured by static incubation in media containing 11.2 mM Glc for 1.5 h. Data represent the mean ± S.E.M of 3 independent experiments. * p ≤ 0.01.