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. Author manuscript; available in PMC: 2013 Jul 3.
Published in final edited form as: Cell Metab. 2012 Jul 3;16(1):33–43. doi: 10.1016/j.cmet.2012.05.011

Figure 6. TLQP-21 reduces apoptosis caused by multiple agents.

Figure 6

(A-C) Primary rat islets were pre-treated for 24 h with DMSO, TLQP-21 (100nM) or Ex-4 (20nM) followed by overnight incubation with thapsigargin (1 μM). Non-thapsigargin treated (control) islets were also included. Following incubation, islet cells were dispersed onto coverslips and stained for nicked DNA using TUNEL (red). Insulin (green) and DAPI (blue) were used as counter stains. Representative micrographs of islets that were pretreated with either DMSO (A) or TLQP-21 (B) followed by thapsigargin treatment are shown. (C) Percentage of TUNEL positive nuclei were determined (~1700 nuclei per experiment) using ImageJ software. (D) Rat islets were treated with etoposide (0.2 mM) for 72 h. DMSO, TLQP-21 (50 nM), exendin-4 (20 nM), or forskolin (5 nM) were added to the media (daily) for the duration of the experiment (72 h). Islet cells were assayed for Alexa Fluor 488-conjugated Annexin V staining via flow cytometry. (C, D) Data represent the mean + S.E.M. from 4-6 independent experiments. * p ≤ 0.05.