Table 2.
Rod single-cell recording parameters
| Idark (pA) | Tpeak (ms) | Tintegr (ms) | Sf(n) (μm2/ph) | I1/2 (ph/μm2) | n (I1/2) | τrec (ms) | τD (ms) | |
|---|---|---|---|---|---|---|---|---|
| PhLP1+/+Cre+ (n = 22) | 14.4 ± 0.3 | 161.0 ± 2.7 | 268.7 ± 12.3 | 1.6 × 10−2 ± 7.7 × 10−4 | 32.2 ± 1.1 | 1.31 ± 0.02 | 220.7 ± 17.0 | 169.1 ± 6.7 |
| PhLP1F/FCre+ (n = 23) | 10.6 ± 0.5*** | 125.0 ± 4.5*** | 915.5 ± 63.7*** | 2.9 × 10−3 ± 4.4 × 10−4*** | 378.7 ± 59.5*** | 1.24 ± 0.05 NS | 1107.9 ± 95.7*** | 2354.2 ± 168.8*** |
The following parameters are from the data in Figure 7. Idark, Dark current measured from saturated responses. Time-to-peak (Tpeak) and integration time (Tintegr) refer to responses whose amplitudes were <0.2 Idark and fell within the linear range. Sf(n), normalized dim flash fractional sensitivity (amplitude of dim flash response divided by flash strength and then normalized for the amplitude of saturating response); I1/2, half-saturating light intensity; n (I1/2), Hill coefficient in the Naka–Rushton equation; τrec, time constant of single-exponential decay of dim flash response recovery phase; τD, dominant time constant of recovery after supersaturating flashes determined from the linear fit to time in saturation versus intensity semilog plot (the “Pepperberg constant”). Values are means ± SEM.
NS, p > 0.05 (not significant);
***p < 0.001 (all compared to wild-type values).