Figure 4.
ATRA treatment inhibited Ag-specific Th2-cell responses and had no effect on Foxp3+ Treg population in spleen. Mice were sacrificed at 24 hours after the final challenge. A. Spleenocytes were isolated from spleens and stimulated with OVA323–339 peptide for 72 hours, then intracellular stained with indicated antibodies after stained with the extracellular maker CD4. The percentages of IL-4 and IFN-γ positive CD4 T cells in the spleen were analyzed by FACS. B. Spleenocytes were isolated from spleens stained for the extracellular maker CD4, CD25 and intracellular markers Foxp3 and analyzed by FACS. The percentages of Treg cells (CD25+ Foxp3+) were gated on CD4+ T cells, The results showed that ATRA pretreatment decreased the percentages of IL-4 positive T cells but no effect on Treg cell population. Data represent five mice of each group in three independent experiments.