Figure 2.

Measurements of sarcolemma integrity. (A and B) CK activities were determined in plasma (A) (n = 6 per genotype; ***P < 0.001) and in GC muscle tissue lysates (B) (n = 3 per genotype; *P < 0.05). (C) CK mRNA levels in QF muscle lysates were determined by qPCR relative to the levels of the reference gene HPRT1 and normalized to wt muscle (n = 4 per genotype; *P < 0.05). (D) EBD penetration into EDL muscle tissue was assessed by immunofluorescence microscopy of cryosections from dye-injected mice (n = 3 per genotype, whole cross-sectional areas of two cryosections per genotype were examined). Note EBD-positive fibers in mdx mice. Data in A-C are presented as mean ± SEM.