Skip to main content
. Author manuscript; available in PMC: 2013 Jun 28.
Published in final edited form as: Methods Mol Biol. 2011;767:67–85. doi: 10.1007/978-1-61779-201-4_6

Fig. 3.

Fig. 3

Use of an object marker greatly simplifies identification of early iPSC colonies for picking and passaging. The marker (shown with the arrow in the left panel) simply takes the place of an objective. When a colony is identified (usually by using the 10× objective), the marker is rotated into place and the position of the colony is marked on the bottom of the plate (marked colonies are shown in the right panel). The plate can then be transferred to a BSC for picking and passaging of the marked colonies.