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. Author manuscript; available in PMC: 2013 Jun 28.
Published in final edited form as: Methods Mol Biol. 2011;767:3–13. doi: 10.1007/978-1-61779-201-4_1

Table 1.

Tissue culture module

Equipment Considerations
Biosafety cabinet What class and type?
Most commonly: Class II Type A2
Incubator Water or air jacketed
One gas (CO2 and air)
Two gases (CO2, N2, and air)
Three gasses (CO2, N2, and O2)
Microscope Phase–contrast
Photo port
Water bath 37°C constant
Low-speed centrifuge Refrigeration not required
Vacuum source Portable
Supplied by the building
2-L Erlenmeyer flasks, in series, with in-line filter Collect aspirate and protect vacuum system
Pipet-Aid Automatic pipettor, cordless, rechargeable
Micropipettors 2, 20, 200, and 1,000 μL