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. Author manuscript; available in PMC: 2013 Jun 28.
Published in final edited form as: Methods Mol Biol. 2011;767:391–398. doi: 10.1007/978-1-61779-201-4_28

Table 1.

Table from stem cell technologies giving seeding densities for generating EBs of various sizes

Desired number of hESCs/hiPSCs per EB AggreWell 400 plate eight wells, each with approximately 1,200 microwells per well
Required number of hESCs/hiPSCs per well
50 6 × 104 cells
100 1.2 × 105 cells
200 2.4 × 105 cells
500 6 × 105 cells
1,000 1.2 × 106 cells
2,000 2.4 × 106 cells
3,000 3.6 × 106 cells
4,000 4.8 × 106 cells