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. Author manuscript; available in PMC: 2013 Jun 28.
Published in final edited form as: Cancer Res. 2008 Oct 1;68(19):8031–8038. doi: 10.1158/0008-5472.CAN-08-1490

Figure 5. Vitamin C has minimal effects on intracellular ROS and has a different effect on cytotoxicity than N-acetyl cysteine.

Figure 5

K562 and RL cells were either untreated (−) or treated (+) to internal vitamin C (AA) concentrations of 18 mM and 8.5 mM respectively, and treated with antineoplastic agents for 6 hours. ROS was measured by incubation with 0.1μg/mL CM-H2DCFDA (A). Results are expressed as median fluorescence intensity (arbitrary units). K562 and RL cells were treated with antineoplastic agents for 48 hours in the presence (+) or absence (−) of 25 mM N-acetylcysteine (NAC) (B). Cell viability was measured by Trypan Blue exclusion and results are expressed as a percent of cells not concomitantly treated with NAC. Three separate experiments were conducted in triplicate and the results shown represent the mean values and the standard deviations of a representative experiment.