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. Author manuscript; available in PMC: 2013 Jun 28.
Published in final edited form as: Methods Mol Biol. 2011;767:201–220. doi: 10.1007/978-1-61779-201-4_15

Fig. 3.

Fig. 3

Adjusting the brightness/contrast of an image. (a) By using the histogram to adjust brightness/contrast levels, you can more accurately adjust the intensity of the image without altering the data. (b) Examples for images that have been appropriately and inappropriately adjusted.