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. 2013 Mar 24;13:28. doi: 10.1186/1475-2867-13-28

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Copyright © 2013 Guo et al.; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Flow cytometric determination of the effect of MIF siRNA on Apoptosis of H460 cells. (A); Cultured H460 cells were divided into three groups: A: cells transfected with NC siRNA, (B) and (C); cells transfected with MIF siRNA (siRNA1 or siRNA2 respectively). After a 48 h treatment, the cells were harvested for quantitation of apoptosis by determining changes in the cell surface expression of Annexin-V. (B) shows a description of the observed frequency of cells undergoing apoptosis which were found to be much higher in the MIF siRNA1 (18.09 ± 0.41%) and MIF siRNA2 (23.38 ± 2.67%) treated groups than in the negative control (NC) treated group (5.87 ± 1.05 and p < 0.05) of H460 cells respectively.