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Phagocytosis assay was performed following pre-treatment of microglial cells with (A) cytochalasin D (10 µM, 30 minutes), (B) fucoidan (500 µg/ml, 30 minutes), (C) wortmannin (10 µM, 60 minutes) and (D) co-incubation with fucoidan and wortmannin. All experiments were performed at least three times independently, and values are normalized to PrP106-126 A117V fibril uptake.
