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. 2013 Jun 28;8(6):e67542. doi: 10.1371/journal.pone.0067542

Figure 1. Knockdown of PKM2 promoted the migration and invasion of BGC823 and SGC7901 cells.

Figure 1

(A) BGC823, SGC7901 and AGS cells were stably transfected with shRNA directed against PKM2. The specific knockdown of PKM2 was monitored by immunoblot (bottom). Cells stably transfected with pcPUR+U6-siPKM2 are referred to as siPK, and those transfected with pcPUR+U6-siRenilla are referred to as pu6. (B) The proliferation of the stably transfected cells. The cell number was determined with the CCK-8 assay, and the relative number of cells is shown. (C, D) A cross-shaped wound was created in the monolayer, and the BGC823 and SGC7901 stably transfected cells were cultured for an additional 24 hours with EGF (100 ng/ml). Representative images of the wounded region are shown. The results of the migration assay are also shown as graphs (*p<0.05). (E, F) The invasion potential of the BGC823 and SGC7901 stable cells was assessed using the BD transwell chamber assay with 100 ng/ml EGF in the lower chamber for 36 hours. The cells that migrated to the lower side of the filter were stained, photographed, and counted. The data are expressed as the mean ± SD from three independent experiments (*p<0.05).