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. 2013 Jun 28;8(6):e68197. doi: 10.1371/journal.pone.0068197

Figure 2. PA increases ACS activity and ACSL3 mRNA level in HASMC.

Figure 2

HASMC were treated with PA (250 µM) for 1 day in the presence or absence of EPA (15 µM). A, ACS activity in cell homogenates was determined as described in Materials and Methods. B, Expression of ACSL1, 3 and 4 mRNAs was determined by real-time PCR and normalized to GAPDH. C, The expression of ACSL1, 3 and 4 protein was determined by Western blot analysis. All values expressed by AU are presented as the mean ± S.E. (n = 3–9). ##p<0.01, ###p<0.001 vs. control, *p<0.05, **p<0.01, ***p<0.001 vs. PA (except for C).