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. 2013 Jun 28;8(6):e68197. doi: 10.1371/journal.pone.0068197

Figure 3. PA increases mRNA level for ACSL3 prior to that for osteoblastic genes in HASMC.

Figure 3

HASMC were treated with PA (250 µM) with or without EPA (15 µM). Expressions of ACSL3 (A), BMP-2 (B), Msx2 (C) and OPN (D). The mRNA levels at 6, 12 and 24 hours after treatment with PA and EPA were determined by real-time PCR and normalized to GAPDH. The control values are shown as 1.0. All values expressed by AU are presented as the mean ± S.E. (n = 4). ###p<0.001 vs. control, *p<0.05, **p<0.01, ***p<0.001 vs. PA.