A, HASMC were transfected with either ACSL3 siRNA or nonsilencing negative control (NC) siRNA, and then cells were treated with PA (250 µM) for 1 day. Expressions of ACSL3, BMP-2, Msx2 and OPN mRNA were determined by real-time PCR and normalized to GAPDH. B, HASMC were infected with Ad-LacZ or Ad-ACSL3 and then treated with PA (250 µM) for 1 day. Expression of ACSL3, BMP-2, Msx2 and OPN mRNAs were determined by real-time PCR and normalized to GAPDH. The control values are shown as 1.0. All values expressed by AU are presented as the mean ± S.E. (n = 4–6). ##p<0.01, ###p<0.001 vs. control+NC siRNA or control+Ad-LacZ, *p<0.05, ***p<0.001 vs. PA+NC siRNA or PA+Ad-LacZ.