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. 2013 Jun 28;8(6):e68197. doi: 10.1371/journal.pone.0068197

Figure 5. PA induces NF-κB activation.

Figure 5

A, HASMC were treated with PA (250 µM) for 1 day with or without EPA (15 µM). Either ACSL3 siRNA or nonsilencing control (NC) siRNA was transfected before PA treatment. Expression of nuclear phospho-NF-κB (p65) protein was determined by Western blot analysis. B, HASMC were transfected with pNF-κB-Luc and then treated with PA (250 µM) for 1 day with or without EPA (15 µM) or Tri C (2 µM). The luciferase activity of the NF-κB reporter was measured according to the manufacturer’s protocol. All values expressed by AU are presented as the mean ± S.E. (n = 6). ###p<0.001 vs. control, **p<0.01, ***p<0.001 vs. PA.